Fig. 3

Absolute and relative H3 acetylation levels in the liver of the Syrian hamster measured by LC-MS/MS. A Overall acetylation of the H3(18–26) peptide (KQLATKAAR). Unacetylated, single, or double acetylated H3(18–26) levels are expressed as a percentage of the total. B Relative levels of the unacetylated, single, or double acetylated H3(18–26) peptide. C Relative site-specific acetylation levels for H3K18, H3K23, H3K27, and H3K79. Levels are relative to the mean of SE. D Lysine-specific acetylation of H3 lysines. H3K18 and H3K23 are determined using one H3 peptide (K₁₈QLATK₂₃AAR); H3K27 is the only acetylated site within the assessed peptide K₂₇SAPATGGVK₃₆K₃₇PHR; as is H3K79 in the peptide EIAQDFK₇₉TDLR. Measurements were performed for summer euthermic (SE), torpor late (TL), and arousal late (AL) animals. All relative levels are calculated compared to the mean of SE. Statistical analysis was performed using Kruskal-Wallis with Dunn’s correction *P <0.05, **P < 0.01, ****P < 0.0001