Long-term aspirin use and epigenetic mitotic clocks for cancer risk prediction: findings in healthy colon mucosa and recommendations for future epigenetic aging studies

Table 3 Baseline versus 10-year follow-up comparisons of median mitotic clock intrinsic rate residual differences between non-users and long-term aspirin users

Measure	Median	P value	Min	Max	IQR
Proximal EpiTOC IR (DNAm)
Non-user T2-T1	− 0.0001		− 0.0005	0.0003	0.0004
Long-term user T2-T1	− 0.0003	0.26	− 0.001	0.0004	0.0003
Distal EpiTOC IR (DNAm)
Non-user T2-T1	− 0.0003		− 0.0008	0.0004	0.0005
Long-term user T2-T1	− 0.00007	0.71	− 0.0009	0.0004	0.0004
Proximal EpiTOC2 IR (cell divisions)
Non-user T2-T1	− 1.1		− 22.8	14.1	18.8
Long-term user T2-T1	− 14.8	0.19	− 69.1	20.7	23.4
Distal EpiTOC2 IR (cell divisions)
Non-user T2-T1	− 11.0		− 40.3	20.7	26.8
Long-term user T2-T1	− 6.2	0.75	− 42.5	23.1	14.8
Proximal MiAge IR (cell divisions)
Non-user T2-T1	− 0.5		− 7.7	1.5	5.1
Long-term user T2-T1	− 4.0	0.23	− 14.8	3.6	4.0
Distal MiAge IR (cell divisions)
Non-user T2-T1	− 0.5		− 7.7	1.5	5.1
Long-term user T2-T1	− 1.6	0.96	− 8.5	2.8	4.3

P values are from Wilcoxon rank sum tests comparing differences between the two aspirin use groups
P values < 0.05 are italicized
T1, timepoint 1 (baseline). T2, timepoint 2 (10-year follow-up)

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