Long-term aspirin use and epigenetic mitotic clocks for cancer risk prediction: findings in healthy colon mucosa and recommendations for future epigenetic aging studies

Table 2 Baseline versus 10-year follow-up within group comparisons of median mitotic clock intrinsic rate (IR) residuals for non-users and long-term aspirin users

Measure	Median	P value	Min	Max	IQR
Proximal EpiTOC IR (DNAm)
Non-user T1	0.0001	–	− 0.0002	0.0005	0.0004
Non-user T2	0.000005	0.12	− 0.0004	0.0002	0.0003
Long-term user T1	0.0002	–	− 0.0005	0.001	0.0004
Long-term user T2	− 0.0002	0.008	− 0.0006	0.0005	0.0002
Distal EpiTOC IR (DNAm)
Non-user T1	0.0001	–	− 0.0005	0.0003	0.0003
Non-user T2	− 0.0001	0.08	− 0.0005	0.00006	0.0003
Long-term user T1	0.0001	–	− 0.0007	0.0007	0.0004
Long-term user T2	0.00001	0.21	− 0.0006	0.0004	0.0003
Proximal EpiTOC2 IR (cell divisions)
Non-user T1	5.4	–	− 11.3	25.8	18.9
Non-user T2	− 0.2	0.28	− 17.4	15.6	14.2
Long-term user T1	7.5	–	− 26.0	53.8	20.4
Long-term user T2	− 8.5	0.009	− 36.3	23.9	14.9
Distal EpiTOC2 IR (cell divisions)
Non-user T1	6.4	–	− 25.4	16.0	15.1
Non-user T2	− 4.7	0.07	− 28.9	3.3	15.7
Long-term user T1	7.3	–	− 36.5	35.5	18.3
Long-term user T2	− 2.3	0.10	− 32.2	14.1	16.3
Proximal MiAge IR (cell divisions)
Non-user T1	0.1	–	− 3.1	7.9	3.7
Non-user T2	− 0.4	0.24	− 4.6	2.5	2.5
Long-term user T1	0.6	–	− 2.6	12.1	4.1
Long-term user T2	− 2.7	0.002	− 6.5	3.4	1.1
Distal MiAge IR (cell divisions)
Non-user T1	0.4	–	− 3.8	4.4	3.3
Non-user T2	− 1.0	0.24	− 8.2	2.8	3.5
Long-term user T1	2.2	–	− 4.5	6.5	3.5
Long-term user T2	− 0.7	0.009	− 6.0	3.1	4.2

P values are from paired Wilcoxon signed rank tests comparing T2 and T1 within aspirin use groups
P values < 0.05 are italicized
T1, timepoint 1 (baseline). T2, timepoint 2 (10-year follow-up)

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