A longitudinal epigenome-wide association study of preeclamptic and normotensive pregnancy

Table 5 Replication phase results examining trimester 3 DNAm data in the discovery (i.e., validation), replication, and combined samples

Replication phase	N/n_control/n_case	N_pairs^*	Mean DNA methylation as beta-values (%)		Mean DNA methylation as M-values		Direction (Cases vs. Controls)	p^**
Replication phase	N/n_control/n_case	N_pairs^*	Controls	Cases	Controls	Cases	Direction (Cases vs. Controls)	p^**
chr6:111902626
Validation sample	53/28/25	25	72.24	78.93	1.41	1.94	+	9.53×10^-5
Independent sample	112/50/62	43	75.16	73.14	1.67	1.49	−	0.11
Combined sample	165/78/87	68	74.11	74.81	1.58	1.62	+	0.65
cg16155413, chr6:111902611
Validation sample	51/28/23	23	46.20	56.34	−0.22	0.37	+	1.05×10^-4
Independent sample	93/38/55	27	53.04	48.73	0.18	−0.08	−	0.06
Combined sample	144/66/78	50	50.13	50.98	0.007	0.05	+	0.68
cg21882990, chr6:111902385
Validation sample	50/26/24	22	81.10	82.05	2.11	2.20	+	0.04
Independent sample	77/30/47	25	80.63	79.47	2.08	1.99	−	0.27
Combined sample	127/56/71	47	80.85	80.34	2.09	2.06	−	0.54

Validation sample, all trimester 3 samples from the discovery phase were sent for validation with the pyrosequencing platform; Independent sample, trimester 3 samples from test sample completely independent of the discovery phase; Combined sample, pooled validation and independent samples
^*Number of matched pairs for which both observations were present
^**P values from the partially overlapping samples t test of M values

ISSN: 2730-7034