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Fig. 4 | Epigenetics Communications

Fig. 4

From: Leukocyte-specific DNA methylation biomarkers and their implication for pathological epigenetic analysis

Fig. 4

Deconvolution of the cellular components of saliva. a–c light microscopy images of H&E stained a salivary leukocytes (salivary leukocytes are labelled i), b buccal epithelial cells, and c mixed saliva (mononuclear cells are labelled ii, and polymorphonuclear cells are labelled iii). d Example of methylation heatmaps for isolated salivary leukocytes and buccal epithelium. Each row represents an individual read, and each column is a CpG site within the amplicon; column names refer to the CpG position in the read. e Density plot (as in Fig. 3c) for the number of methylated CpG sites per read in isolated buccal epithelium (blue) and salivary leukocyte (red) samples (bandwidth = 0.45). f Methylation heatmaps (as explained in Fig. 3b) for mixed saliva samples performed in triplicate. g Scatter plot of observed read classification from methylation analysis vs expected read classification from microscopic measurement of the proportion of salivary leukocytes

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