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Table 2 Association results of ANGPT1 methylation and DCI in the EWAS (450K) and the replication (MethylSeq) analyses

From: ANGPT1 methylation and delayed cerebral ischemia in aneurysmal subarachnoid hemorrhage patients

  

450K

MethylSeq

CpG site

Positiona

Discoveryb (N = 68)

Replication (N = 175)

Discovery + replication (N = 233)

Coefficient

95% CI

p-value

Coefficientc

95% CI

p-valued

Coefficientc

95% CI

p-valued

cg09396217

chr8:108510286

0.044

− 0.135, 0.222

0.63

− 0.215

− 0.389, − 0.040

0.016

− 0.185

− 0.333, − 0.038

0.014

cg18031596

chr8:108510292

0.398

0.244, 0.552

3.35 × 10−6

− 0.200

− 0.369, − 0.030

0.021

− 0.145

− 0.293, 0.003

0.054

cg27616227

chr8:108510314

0.189

0.009, 0.368

0.04

− 0.149

− 0.290, − 0.009

0.038

− 0.123

− 0.240, − 0.006

0.040

chr8:108510324

chr8:108510324

-

 

-

− 0.177

− 0.327, − 0.027

0.021

− 0.176

− 0.297, − 0.056

0.004

cg02536838

chr8:108510343

0.301

0.147, 0.455

2.52 × 10−4

− 0.116

− 0.242, 0.011

0.073

− 0.097

− 0.205, 0.011

0.077

  1. aGRCh37.p13
  2. bModel M ~ DCI + age + sex + surrogate variables. The coefficients, CIs, and p-values under the “450K” meta-column are different from the EWAS results because we used a different statistical method for model parameter estimation in order to be consistent with the method used for analyzing the MethylSeq data (those shown under the “MethylSeq” meta-column). The purpose was to make the results more directly comparable
  3. cModel M ~ DCI + age + sex. Represents the average difference in M value between cases and controls, with control being the reference
  4. dBold p-values are less than the Bonferroni significance threshold for replication analysis: p-value < 0.05/1.1 = 0.045